Native acridone synthases I and II from Ruta graveolens L. form homodimers
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چکیده
منابع مشابه
Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L.
Acridone synthase has been purified from cell suspension cultures of Ruta graveolens using a combination of gel filtration and ion exchange chromatography. The purified enzyme has an apparent molecular weight of 69 kDa on gel filtration and a subunit structure on SDS-PAGE of 40 kDa. The apparent Km-values are 10.64 microM and 32.8 microM for N-methylanthraniloyl-CoA and malonyl-CoA, respectivel...
متن کاملBiosynthesis of Acridone Alkaloids. A Cell-Free System from Ruta graveolens Cell Suspension Cultures
Ruta graveolens, Suspension Cultures, Cell-Free System, Acridone Biosynthesis, 1,3-DihydroxyN-methylacridone A cell-free extract of Ruta graveolens cell suspension cultures, strain R-20, was prepared which synthesizes 1,3-dihydroxy-N-methylacridone from N-methylanthranilic acid and malonylcoenzyme A. The reaction is strongly dependent on the presence of ATP. After adding of coenzyme A to the as...
متن کامل[Acridine-alkaloids from callus cultures of Ruta graveolens L].
Rutacridone, 1-hydroxy-A-methylacridone, and l-hydroxy-3-methoxy-A-methylacridone have been isolated for the first time from callus cultures, which had been obtained from meristematic stem cells of Ruta graveolens L. The alkaloids were identified by co-chromatography with authentic compounds, by IR spectra, and by electronic spectra before and after addition of A1C13 . The use of the bathochrom...
متن کاملVolatile Composition of Ruta graveolens L. of North of Iran
The chemical constituents of the essential oil of Ruta graveolens L. from aerial parts collected from Iran were analyzed by GC and GC/MS. The oil yield of the dried plant obtained by hydrodistillation was 0.4% (v/w). Nineteen compounds, representing for 95.2% of the oil, were identified. The main classes of compounds were found to be ketons(46.6%) and sesquiterpenoids (13.3%) and monoterpenoids...
متن کاملRutacridone-epoxide, a New Acridone Alkaloid from Rut a graveolens *
Several acridone alkaloids have been isolated from roots of Ruta graveolens (for summary see [1, 2]). One of the main alkaloids is rutacridone (1) which seems to be a precursor of several derivatives with hydroxyl or chlorine functions at the isopropyl moiety. As an intermediate an 18,19-epoxide of rutacridone could be postulated [1, 3] that has not yet been identified. Screening for antimicrob...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1999
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)00355-5